垂直電泳槽 Thermo Scientific Owl P10DS Dual Gel System | | Code | NM0280-0010 | Classification | 電泳相關設備 | Size | | Order | |
Print |
|
| PRODUCT DETAIL |
Thermo Scientific Owl P10DS Dual Gel System
Gel Size: (W x L) 20 x 20 cm
Footprint: (W x L x H) 31 x 16.50 x 23 cm
UBC Vol: 650 ml
LBC Vol: 500 ml
Total Running Buffer Volume: 1150 ml
The optimal device for a large number of samples that need extended separation and mobility shift assays.
Our Owl P10DS Dual Gel System casts and runs 1 or 2 (W x L) 20 x 20 cm gels in the upper buffer chamber. The larger size cassette gives you the maximum number of samples and separation length. Ideal for second dimension protocols of 2-D electrophoresis applications.
Our Owl P10DS Dual Gel System is manufactured using Owl’s time tested acrylic fabrication technology providing a durable, long lasting system backed by a three year warranty. The design allows for a very simple clamping mechanism that makes loading glass cassettes and most manufacturer’s pre cast gels trouble free.
|
P10DS-2 Gel Casting Instructions |
Casting with Owl's Emperor Penguin™ System |
|
|
1. Load the glass cassettes with the simple clamping mechanism |
|
|
|
|
|
|
|
2. Pour an agarose plug in the specially designed agarose well |
|
|
|
|
|
|
|
3. Cast the acrylamide solution in the cassettes and once solidified, load your samples. |
|
|
|
|
|
|
|
|
P10DS-2 Sample Gel |
P10DS-2 Sample Gel |
|
|
|
12% ProSieve® 50 Gels, 10 well, 19x20cm 1X Tris-Glycine-SDS running buffer, 150V for 75 minutes Samples consisted of ProSieve® Protein markers (BMA Cat. No. 50547) and chemical or ultrasonic extraction of soluble proteins from the following E. coli varients: E. coli DH5a, E. coli LE392, E. coli TM101 and E. coli ATCC 25922 in repeating sequence (1, 2, 3, 4, 5, 1, 2, 3, 4, 5, etc.). Concentration of sample is approximately 2.5-3.0ug/lane. Stain with Coommassie Brilliant Blue R-250, destain with acid-alcohol. Data provided by BioWhittaker Molecular Applications, Inc.
|
|
|
|
Comb Options |
Catalog
Number |
Comb
Type
|
Number
of Teeth
|
Thickness
of Teeth
|
Width
of Teeth
|
EST Well
Volume |
P2-10C |
Standard Comb |
10 |
0.8mm |
13.6mm |
239µl |
P2-10D |
Standard Comb |
10 |
1.5mm |
13.6mm |
449µl |
P2-15C |
Standard Comb |
15 |
0.8mm |
8.2mm |
144µl |
P2-15D |
Standard Comb |
15 |
1.5mm |
8.2mm |
271µl |
P2-20C |
Standard Comb |
20 |
0.8mm |
5.5mm |
97µl |
P2-20D |
Standard Comb |
20 |
1.5mm |
5.5mm |
182µl |
P2-25C |
Standard Comb |
25 |
0.8mm |
3.9mm |
69µl |
P2-25D |
Standard Comb |
25 |
1.5mm |
3.9mm |
129µl |
P2-PREP |
Prep |
2 |
1.5mm |
148/4.7 |
488/155µl |
Complete System Includes:
• Upper Buffer Chamber with Internal Cooling Core
• Lower Buffer Chamber
• SuperSafe Lid with Attached Power Supply Leads
• 4 Blank Glass Plates
• 4 Notched Glass Plates
• 4 Combs: (2) 15 Well & (2) 20 Well, 1.5 mm Thick
• Spacer Set: 1.5 mm Thick
• Blocking Plate for Single Gel Operation
• Spacer Placer
Illustration of Complete System
|
Ordering Guide |
|
Item Description |
Cat. No. |
Complete System |
P10DS-2 |
Accessories |
Cat. No. |
Thermo Scientific Owl JGC-3 Gel Casting System
Gel Size: (W x L) 20 x 20 cm
Footprint: (W x L x H) 23 x 17 x 17 cm
Compatible with Owl P10DS |
JGC-3 |
Offset Glass, (W x L x H) 20 x 18 x 0.32 cm |
P10-18G |
Blank Glass, (W x L x H) 20 x 20 x 0.32 cm |
P10-20G |
Notched Glass, (W x L x H) 20 x 20 x 0.32 cm |
P10-20R |
Side Clamps (1 pair.) |
P10-CL |
Upper Buffer Chamber Assembly |
P10DS-001 |
Blocking Plate |
P10DS-006 |
Lower Buffer Chamber |
P10DS-008 |
SuperSafe Lid with Attached Power Supply Leads |
P10DS-012 |
Owl P10DS-2 with Owl JGC-3 Casting System |
P10DS-1 |
P10DS-2 Apparatus Only |
P10DS-3 |
Replacement Gaskets (1pair.) |
P10-GK |
Offset Glass Offset Glass, (W x L x H) 20 x 18 x 0.48 cm |
P2-18G |
Frosted Blank Glass,(W x L x H) 20 x 20 x 0.48 cm |
P2-20FG |
Frosted Notched Glass, (W x L x H) 20 x 20 x 0.48 cm |
P2-20FR |
Blank Glass, (W x L x H) 20 x 20 x 0.48 cm |
P2-20G |
Notched Glass, (W x L x H) 20 x 20 x 0.48 cm |
P2-20R |
Thermo Scientific Owl P2-CST Multiple Gradient Gel Caster
Gel Size: (W x L) 20 x 20 cm
Footprint: (W x L x H) 25 x 13 x 23 cm
Compatible with Owl P10DS |
P2-CST |
Spacer Set, (W x H) 1 x 0.08 cm |
P2-SC |
1.0 x 1.5 mm Spacer Set |
P2-SD |
Thermo Scientific Owl Power Supply Leads |
PSL-5 |
Wing Knob |
R10446 |
Sponge Tips (Pkg. of 10.) |
ST-10 |
Combs |
Cat. No. |
Standard Comb |
P2-10C |
Standard Comb |
P2-10D |
Standard Comb |
P2-15C |
Standard Comb |
P2-15D |
Standard Comb |
P2-20C |
Standard Comb |
P2-20D |
Standard Comb |
P2-25C |
Standard Comb |
P2-25D |
Prep |
P2-PREP |
|
|
|
| |