水平電泳槽 Owl Horizontal Electrophoresis -A6 Wide Gel‚ 23 x 25 cm
Code  NM0200-0010
Classification  電泳相關設備
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PRODUCT DETAIL

Thermo Scientific A6 Wide Gel System
Gel Size: (W x L) 23 x 25 cm
Footprint: (W x L x H) 38 x 35 x 11 cm
Running Buffer Volume: 3000 ml
Great for high throughput laboratories.

Our Owl A6 Wide Gel System offers a simple, convenient and fast method for screening multiple samples on a single agarose gel. 25 to 500 samples can be run simultaneously on one gel; producing clear, tight banding patterns with no "smiling".

Our Owl A6 Wide Gel System incorporates Owl’s end gate casting design. Each side of the UVT gel tray is fitted with gasketed end gates which provide a leakproof seal without tape. Built in leveling ensures the casting of flat, uniformly thick gels. Sample loading is greatly enhanced using micro well pipette format combs (and a multichannel pipette) engineered specifically for use with this system. Using these combs and a multichannel pipette, samples may be loaded directly from a 96-well plate, 8 or 12 at a time. Sample loading with a multichannel pipette speeds up transfer and reduces the chance of sample order error.

A6 Gel Casting Instructions
Gel Casting Using Owl's End Gate Casting System
1. Place the two end gates into the tray with the gasket side facing out.
2. Prepare and pour warm (>60o) agarose solution onto the UVT gel tray (s).
3. Place combs into slots provided on the walls of the UVT gel tray.
4. Remove the end gates, place the UVT gel tray in the buffer chamber, add buffer, load samples, and run the gel.

A6 Sample Gel
2% SeaKem® Gold agarose (BMA Cat. No. 50150) in 1X TBE, pH 8.2 and 0.5ug/mL ethidium bromide, using an Owl Gel Casting Array with 50 tooth combs. Samples consisted of 50-100bp DNA size marker (BMA No. 50461) in the odd wells and a 100bp DNA ladder (BMA Cat. No. 50321) in the even wells. Both sample at a concentration of approximately 25ng/band adjusted for well volume. The gel was run for approximately 30 minutes at 7V/cm Data provided by BioWhittaker Molecular Applications, Inc.

Comb Options
Catalog
Number
 Comb
Type
 Number
of Teeth
 Thickness
of Teeth
 Width
of Teeth		 Recommended Loading Volumes
0.25cm 0.5cm 0.75cm 1.0cm
D3-MT2C Micro Well 50 (2X) 1.0mm 3mm  2µl 8µl 14µl  19µl 
D3-MT2D Micro Well 50 (2X) 1.5mm 3mm  3µl  12µl  20µl 29µl 
D3-MTC Micro Well 25 (1X)  1.0mm 7.5mm  6µl  20µl    48µl 
D3-MTD Micro Well 25 (1X)  1.5mm 7.5mm  8µl  30µl  51µl  72µl 
D3-WALL Wall 1 1.5mm 230         

Complete System Includes:
• Buffer Chamber with Three Point Leveling Base
• SuperSafe Lid with Attached Power Supply Leads
• U.V. Transmissible (UVT) Gel Tray with Gasketed End Gates
• 4 Combs: (2) 25 Well and (2) 50 Well, 1.5 mm Thick

Illustration of Complete System
illustration

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Ordering Guide
Item Description Cat. No.
Complete System A6

Accessories Cat. No.
Replacement End Gates (1 pair.) A3-1-EG
Replacement Gaskets (1 pair.) A3-GK-1
UVT Gel Tray with Gasketed End Gates A6-UVT
Casting Dam, (L) 23 cm DAM-23
Thermo Scientific Owl Power Supply Leads PSL-5

Combs Cat. No.
Micro Well D3-MT2C
Micro Well D3-MT2D
Micro Well D3-MTC
Micro Well D3-MTD
Wall D3-WALL