GAGTCNNNN↓NN

CTCAGNNNNNNN

Purified from Bacillus stearothermophilus

Concentration:
2-10000 units/ml

Activity assay:
Phage T7 DNA, SE buffer N.BstT9, 55℃

Recommended buffer:
N.BstT9

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Heat inactivation: 20 min, 65℃ - No;  20 min, 80℃ - Yes