ATGCA↓T 

T↑ACGTA

Purified from Zoogloea species 2

Isoschizomer Nsi I

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 20 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes