Download: Datasheet Vsp I

AT↓TAAT

TAAT↑TA 

Purified from Vibrio species 343

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37℃

Recommended buffer:
W

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 10 units of the enzyme at 37℃.

Heat inactivation  (20 min, 65℃):
Yes