Download: Datasheet Tru9 I

T↓TAA 

AAT↑T

Purified from Thermus rubber 9

Concentration:
20000-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 65℃

Recommended buffer:
W

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 95% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 40 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃): 
No