Download: Datasheet Taq I

T↓CGA

AGC↑T

Purified from Thermus aquaticus

Concentration:
5000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 65℃

Recommended buffer:
Y

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 6 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No