Download: Datasheet Sse9 I (Tsp509 I)

↓AATT

TTAA↑

Purified from Sporosarcina species 9

Concentration:
1-5000 units/ml

Activity assay:
Phage λ  DNA, SE buffer B, 55℃

Recommended buffer:
B

Ligation/recutting assay:
After 2-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 8 units of the enzyme at 55℃

Heat inactivation (20 min, 65℃):
Yes