Download: Datasheet Sph I

GCATG↓C

C↑GTACG

Purified from Sphaerotilus species

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 10 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes