Download: Datasheet Smi I (Swa I)

ATTT↓AAAT

TAAA↑TTTA

Purified from Streptococcus milleri S

Isoschizomer Swa I

Concentration:
20-50000 units/ml

Activity assay:
Phage T7 DNA/Ssp I digest, SE buffer O, 37℃

Recommended buffer:
O

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 80% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 40 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes