Download: Datasheet Sma I

CCC↓GGG

GGG↑CCC

Purified from E.coli strain that carries Sma I gene from Serratia marcenscens

Concentration:
20000 units/ml

Activity assay:
Phage λ DNA/Hind III digest, SE buffer Y, 25℃.

Recommended buffer:
Y

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut by T4 DNA ligase together with 10% of PEG.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 40 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes