Download: Datasheet Psp 124B I (Sac I)

GAGCT↓C

C↑TCGAG

Purified from Pseudomonas species 124B

Isoschizomer Sac I

Concentration:
20000-50000 units/ml

Activity assay:
Phage λ DNA/Hind III, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:
After 40-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 40 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No