Download: Datasheet Ple19 I

CGAT↓CG

GC↑TAGC

Purified from Pseudomonas lemoignei 19

Isoschizomer Pvu I

Concentration:
5000 units/ml

Activity assay:
Phage λ  DNA/Hind III, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:
After 2-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 10 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃) :
Yes