Download: Datasheet Pci I (BspLU11 I)

A↓CATGT

TGTAC↑A

Purified from Planococcus citreus SE-F45

Isoschizomer BspLU11 I

Concentration:
10000 units/ml

Activity assay:
Phage T7 DNA, SE buffer O, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of  DNA with 20 units of the enzyme at 37℃.

Heat inactivation (20 min, 65 ℃):
Yes