Download: Datasheet Pce I (Stu I)

AGG↓CCT

TCC↑GGA  

Purified from Planococcus citreus 55

Isoschizomer Stu I

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 50℃

Recommended buffer:
Y

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of DNA with 20 units of the enzyme at 50℃.

Heat inactivation (20 min, 65℃):
No