Download: Datasheet Nru I

TCG↓CGA

AGC↑GCT

Purified from Nocardia rubra

Concentration:  10000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37℃

Recommended buffer:
W

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 10% of the DNA fragments may be ligated.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 15 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No