Download: Datasheet MroN I (Nae I)

G↓CCGGC

CGGCC↑G

Purified from Micrococcus roseus NO

Concentration:
5000 units/ml

Activity assay:
Adenovirus-2 DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments of pBR322 may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 4 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No