Download: Datasheet Kzo9 I (Mbo I)

↓GATC

CTAG↑

Purified from Kurthia zopfii 9

Isoschizomer Sau3A I

Concentration:
2-5000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 95% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 4 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes