Download: Datasheet Kpn I

GGTAC↓C

C↑CATGG

Purified from Klebsiella pneumonia

Concentration:
20000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
W

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No