Download: Datasheet HspA I (Hha I)

G↓CGC

CGC↑G

Purified from Haemophilus species A1

Isoschizomer Hin6 I

Concentration:
10-30000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No