Download: Datasheet Hinf I

G↓ANTC

CTNA↑C

Purified from Haemophilus influenzae

Concentration:
10-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer O, 37℃

Recommended buffer:
O

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 80 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃) :
No