Download: Datasheet FauND I (Nde I)

CA↓TATG

GTAT↑AC

Purified from Flavobacterium aquatili ND

Isoschizomer Nde I

Concentration:
10-20000 units/ml

Activity assay:
Phage λ DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:
After 3-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 6 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes

Note! The enzyme is very sensitive to DNA impurities.