Download: Datasheet EcoR V

GAT↓ATC

CTA↑TAG

Purified from Escherichia coli

Concentration:
40000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37℃

Recommended buffer:
W

Ligation/recutting assay:
After 20-fold overdigestion with EcoR V, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of  the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No