Download: Datasheet EcoR I

G↓AATTC

CTTAA↑G

Purified from E.coli strain that carries the plasmid with EcoR I gene from Escherichia coli.

Concentration:
20-50000 units/ml

Activity assay:
Phage λ DNA, EcoR I buffer, 37℃

Recommended buffer:
EcoR I

Ligation/recutting assay:
After 40-fold overdigestion with EcoR I, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes