Download: Datasheet Dra I (Aha III)
TTT↓AAA
AAA↑TTT
Purified from Deinococcus radiophilus
Concentration:
10-30000 units/ml
Activity assay:
Phage λ DNA, SE buffer G, 37℃
Recommended buffer:
G
Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of the enzyme at 37℃.
Heat inactivation (20 min, 65 ℃):
No |