Download: Datasheet CciN I (Not I)

GC↓GGCCGC

CGCCGG↑CG

Purified from Curtobacterium citreus N

Isoschizomer Not  I

Concentration:
5000 units/ml

Activity assay:
Adenovirus-2 DNA, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No