Download: Datasheet BsuR I (Hae III)

GG↓CC

CC↑GG

Purified from Bacillus subtilis R

Isoschizomer Hae III

Concentration:
50000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No