Download: Datasheet BstHH I (Hha I)

GCG↓C             

C↑GCG     

Purified from  Bacillus stearothermophilus HH

Isoschizomer Hha I

Concentration:
50000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 50℃

Recommended buffer:
Y

Ligation/recutting assay:
After 40-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃) :
No