Download: Datasheet BstH2 I (Hae II)

RGCGC↓Y   

Y↑CGCGR  

Purified from Bacillus stearothermophilus H2

Isoschizomer Hae II

Concentration: 
10-30000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 65℃

Recommended buffer:
Y

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No