Download: Datasheet BstFN I

CG↓CG          

GC↑GC      

Purified from Bacillus stearothermophilus FN

Concentration:
2-10000 units/ml

Activity assay: 
Phage λ DNA, SE buffer Y, 60℃

Recommended buffer:
Y

Ligation/recutting assay:      
After 10-fold overdigestion with the enzyme, more than 95% of the DNA fragments may be ligated and  recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No