Download: Datasheet BstDS I

C↓CRYGG      

GGYRC↑C

Purified from Bacillus stearothermophilus DS

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 65℃

Recommended buffer:
Y

Ligation/recutting assay: 
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and  recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No