Download: Datasheet Bst4C I

ACN↓GT

TG↑NCA

Purified from Bacillus stearothermophilus 4C

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 65℃

Recommended buffer:
Y

Ligation/recutting assay:      
After 5-fold overdigestion with the enzyme, less than 5% of the DNA fragments may be ligated.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of the enzyme at 65℃.

Heat inactivation (20 min, 65℃):
No