BssT1 I (Sty I)
Code  BO0230-5000
Classification  限制酵素
Size  5000u
Order 加入購物車
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Opens internal link in current windowDownload: Datasheet BssT1 I (Sty I)

C↓CWWGG      

GGWWC↑C 

Purified from Bacillus stearothermophilus T1

Isoschizomer Sty I

Concentration:
20000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 60℃

Recommended buffer:
W

Ligation/recutting assay:      
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):  
No

Note! The excess of the enzyme may cause star activity