Download: Datasheet Bse21 I

CC↓TNAGG

GGANT↑CC

Purified from Bacillus species 21

Concentration:
10-30000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:    
After 2-fold overdigestion with the enzyme, more than 50% of the DNA fragments may be ligated and more than 95% recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):   
No