Download: Datasheet Bse118 I (Cfr10 I)

R↓CCGGY

YGGCC↑R   

Purified from Bacillus stearothermophilus 118

Isoschizomer Cfr10 I

Concentration:
2000 units/ml

Activity assay: 
Phage λ DNA, SE buffer O, 65℃.

Recommended buffer:
O

Ligation/recutting assay:      
After 2-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and  recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 2 units of enzyme at 65℃.

Heat inactivation (20 min, 65℃) :
No