Download: Datasheet Bsc4 (Bsl I)

CCNNNNN↓NNGG

GGNN↑NNNNNCC

Purified from Bacillus schlegelii 4

Isoschizomer Bsl I

Concentration:
20-60000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 55℃.

Recommended buffer:
W

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16h incubation of 1 mg of λ DNA with 60 units of enzyme at 55℃.

Heat inactivation (20 min, 65℃)  :
No