Download: Datasheet Bpu14 I

TT↓CGAA

AAGC↑TT

Purified from Bacillus pumilis 14

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:     
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes