Download: Datasheet Bpu14 I
TT↓CGAA
AAGC↑TT
Purified from Bacillus pumilis 14
Concentration:
10000 units/ml
Activity assay:
Phage λ DNA, SE buffer G, 37℃
Recommended buffer:
G
Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.
Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37℃.
Heat inactivation (20 min, 65℃):
Yes |