Download: Datasheet Bme18 I (Ava II)

G↓GWCC

CCWG↑G

Purified from Bacillus megaterium 18

Isoschizomer Ava II

Concentration:
10000 units/ml.

Activity assay:
Phage λ DNA, SE buffer O, 37℃.

Recommended buffer:
O

Ligation/recutting assay:
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 20 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes