Download: Datasheet Bgl II

A↓GATCT

TCTAG↑A

Purified from Bacillus globigii

Concentration:
10-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer O, 37℃

Recommended buffer:
O

Ligation/recutting assay:     
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:  
No characteristic banding pattern change after 16h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes