Download: Datasheet BamH I

G↓GATCC 

CCTAG­↑G 

Purified from E. coli strain that carries BamH I gene from Bacillus amiloliquefaciens.

Concentration:
50-150000 units/ml

Activity assay:
Phage λ DNA, SE buffer G, 37℃

Recommended buffer:
G

Ligation/recutting assay:      
After 50-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 200 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃): 
Yes