Download: Datasheet AsuNH I (Nhe I)

G↓CTAGC 

CGATC↑G

Purified from Actinobacillus suis NH

Isoschizomer Nhe I

Concentration:
5-20000 units/ml

Activity assay:
Phage λ DNA/Hind III digest, SE buffer B, 37℃.

Recommended buffer:
B

Ligation/recutting assay:
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of the enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes