Download: Datasheet AspS9 I (Sau96 I)

G↓GNCC

CCNG↑G 

Purified from Arthrobacter species S9

Isoschizomer Sau96 I

Concentration:
40000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37℃.

Recommended buffer:
W

Ligation/recutting assay:
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes