Apa I
Code  BO0090-5000
Classification  限制酵素
Size  5000u
Order 加入購物車
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PRODUCT DETAIL

Opens internal link in current windowDownload: Datasheet Apa I

GGGCC↓C

C↑CCGGG  

Purified from Acetobacter pasteurianus

Concentration:
10-30000 units/ml

Activity assay:
Phage λ DNA(dam-)/BamH I digest, SE buffer Y, 37℃

Recommended buffer: 
Y

Ligation/recutting assay:   
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 40 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes  

Note! Cleavage by Apa I may be blocked by overlapping dcm-methylation