Download: Datasheet Ahl I (Spe I)

A↓CTAGT  

TGATC↑A 

Purified from Alteromonas haloplanktis SP

Isoschizomer Spe I

Concentration:
30000 units/ml

Activity assay:
Phage T7 DNA, SE buffer B, 37℃

Recommended buffer:
B

Ligation/recutting assay:      
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 100 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃): 
No