Download: Datasheet Afe I (Eco47 III)

AGC↓GCT 

TCG↑CGA 

Purified from Alcaligenes faecalis T2774

Isoschizomer Eco47 III

Concentration: 
10-50000 units/ml

Activity assay: 
Phage λ DNA/BamHI digest, SE buffer Y, 37℃.

Recommended buffer:
Y

Ligation/recutting assay:      
After 10-fold overdigestion with the enzyme, more than 80% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃): 
Yes