Download: Datasheet Acs I (Apo I)

R↓AATTY

YTTAA↑R

Purified from Arthrobacter citreus

Concentration:
20-50000 units/ml.

Activity assay:
Phage λ DNA, SE buffer W, 37℃

Recommended buffer:
W

Ligation/recutting assay:    
After 20-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 50 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
No