Download: Datasheet AccBS I

GAG↓CGG

CTC↑GCC

Purified from Acetinobacter calcoaceticus BS

Concentration: 
5-20000 units/ml

Activity assay: 
Phage λ DNA, SE buffer Y, 37℃.

Recommended buffer:
Y

Ligation/recutting assay:  
After 5-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes