Download: Datasheet Acc65

G↓GTACC

CCATG↑G 

Purified from Acinetobacter calcoaceticus

Concentration:
30-50000 units/ml

Activity assay:
Phage λ DNA, SE buffer W, 37℃

Recommended buffer:    
W

Ligation/recutting assay:      
After 10-fold overdigestion with the enzyme, more than 90% of the DNA fragments may be ligated and recut

Nonspecific endonucleases test:   
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 30 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):   
Yes

Note! Activity may be blocked by overlapping dcm methylation.