Download Datasheet: Acc113 I (Sca I)

AGT↓ACT

TCA↑TGA

Purified from Acetinobacter calcoaceticus 113

Isoschizomer Sca I

Concentration:
10000 units/ml

Activity assay:
Phage λ DNA, SE buffer Y, 37℃

Recommended buffer:
Y

Ligation/recutting assay:      
After 5-fold overdigestion with the enzyme, more than 70% of the DNA fragments may be ligated and recut.

Nonspecific endonucleases test:    
No characteristic banding pattern change after 16 h incubation of 1 mg of λ DNA with 10 units of enzyme at 37℃.

Heat inactivation (20 min, 65℃):
Yes